Improved Cryptosporidium case findings using immunofluorescent microscopy on concentrated stool

Comment on: Clinical, Histopathological and Immunofluorescent Findings of IgA Nephropathy

Improved stool concentration procedure for detection of Cryptosporidium oocysts in fecal specimens.

Epidemiologic and laboratory data suggest that coprodiagnostic methods may fail to detect Cryptosporidium oocysts in stool specimens of infected patients. To improve the efficacy of stool concentration procedures, we modified different steps of the Formalin-ethyl acetate (FEA) stool concentration technique and evaluated these modifications by examining stool samples seeded with known numbers of...

Comment on: clinical, histopathological and immunofluorescent findings of IgA nephropathy.

We read with interest the recently published article by Tipu et al. entitled “Clinical, histopathological and immunofluorescent findings of IgA nephropathy”, in the esteemed Iranian Journal of Immunology (1). The study described frequency of different clinical, histopathological and immunofluorescent characteristics of IgA nephropathy. The authors should be applauded for their findings; however...

Clinical, Histopathological and Immunofluorescent Findings of IgA Nephropathy

Background: IgA nephropathy, a prevalent disease in Asia, is considered the main cause of end stage renal disease among primary glomerular disease. Objective: To determine the frequency of different clinical, histopathological and immunofluorescent characteristics of IgA nephropathy. Methods: Renal biopsies of 376 patients were received for immunofluorescent and for histopathological studies. B...

Flow cytometric detection of Cryptosporidium oocysts in human stool samples.

Cryptosporidium parvum is an important pathogen that causes diarrhea in virtually all human populations. Improved diagnostic methods are needed to understand the risk factors, modes of transmission, and impact of cryptosporidiosis. In the present study, we fluorescently labeled and counted C. parvum oocysts by flow cytometry (FC) and developed a simple and efficient method of processing human s...